J. Crayton Pruitt Family Department of Biomedical Engineering

UF Diabetes Institute

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Publications

  1. Barra JM, Kratz AT, Castro-Gutierrez R, Proia J, Bhardwaj G, Phelps E, Russ HA. Cryopreservation of stem cell-derived beta-like cells enriches for insulin- producing cells with improved function. Diabetes. 2024 Jul 31:db240346. doi: 10.2337/db24-0346. Epub ahead of print. PMID: 39083654.
  2. Drotar DM, Mojica-Avila AK, Bloss DT, Cohrs CM, Manson CT, Posgai AL, Williams MD, Brusko MA, Phelps EA, Wasserfall CH, Speier S, Atkinson MA. Impaired islet function and normal exocrine enzyme secretion occur with low inter-regional variation in type 1 diabetes. Cell Rep. 2024 Jun 25;43(6):114346. doi: 10.1016/j.celrep.2024.114346. Epub 2024 Jun 7. PMID: 38850534; PMCID: PMC11251461.
  3. Sylvester-Armstrong KR, Reeder CF, Powell A, Becker MW, Hagan DW, Chen J, Mathews CE, Wasserfall CH, Atkinson MA, Egerman R, Phelps EA. Serum from pregnant donors induces human beta cell proliferation. Islets. 2024 Dec 31;16(1):2334044. doi: 10.1080/19382014.2024.2334044. Epub 2024 Mar 27. PMID: 38533763; PMCID: PMC10978022.
  4. Widener AE, Roberts A, Phelps EA. Granular Hydrogels for Harnessing the Immune Response. Adv Healthc Mater. 2023 Dec 25:e2303005. doi: 10.1002/adhm.202303005. Epub ahead of print. PMID: 38145369; PMCID: PMC11196388.
  5. Becker MW, Peters LD, Myint T, Smurlick D, Powell A, Brusko TM, Phelps EA. Immune engineered extracellular vesicles to modulate T cell activation in the context of type 1 diabetes. Sci Adv. 2023 Jun 2;9(22):eadg1082. doi: 10.1126/sciadv.adg1082. Epub 2023 Jun 2. PMID: 37267353; PMCID: PMC10765990.
  6. Widener AE, Roberts A, Phelps EA. Single versus dual microgel species for forming guest-host microporous annealed particle PEG-MAL hydrogel. J Biomed Mater Res A. 2023 Sep;111(9):1379-1389. doi: 10.1002/jbm.a.37540. Epub 2023 Apr 3. PMID: 37010360; PMCID: PMC10909382.
  7. Mastracci TL, Apte M, Amundadottir LT, Alvarsson A, Artandi S, Bellin MD, Bernal-Mizrachi E, Caicedo A, Campbell-Thompson M, Cruz-Monserrate Z, El Ouaamari A, Gaulton KJ, Geisz A, Goodarzi MO, Hara M, Hull-Meichle RL, Kleger A, Klein AP, Kopp JL, Kulkarni RN, Muzumdar MD, Naren AP, Oakes SA, Olesen SS, Phelps EA, Powers AC, Stabler CL, Tirkes T, Whitcomb DC, Yadav D, Yong J, Zaghloul NA, Pandol SJ, Sander M. Integrated Physiology of the Exocrine and Endocrine Compartments in Pancreatic Diseases: Workshop Proceedings. Diabetes. 2023 Apr 1;72(4):433-448. doi: 10.2337/db22-0942. PMID: 36940317; PMCID: PMC10033248.
  8. Santini-González J, Castro-Gutierrez R, Becker MW, Rancourt C, Russ HA, Phelps EA. Human stem cell derived beta-like cells engineered to present PD-L1 improve transplant survival in NOD mice carrying human HLA class I. Front Endocrinol (Lausanne). 2022 Nov 25;13:989815. doi: 10.3389/fendo.2022.989815. PMID: 36506044; PMCID: PMC9732725.
  9. Widener AE, Duraivel S, Angelini TE, Phelps EA. Injectable Microporous Annealed Particle Hydrogel Based on Guest-Host-Interlinked Polyethylene Glycol Maleimide Microgels. Adv Nanobiomed Res. 2022 Oct;2(10):2200030. doi: 10.1002/anbr.202200030. Epub 2022 Aug 21. PMID: 36419640; PMCID: PMC9678130.
  10. Hagan DW, Ferreira SM, Santos GJ, Phelps EA. The role of GABA in islet function. Front Endocrinol (Lausanne). 2022 Sep 29;13:972115. doi: 10.3389/fendo.2022.972115. PMID: 36246925; PMCID: PMC9558271.
  11. Stokes C, Pino JA, Hagan DW, Torres GE, Phelps EA, Horenstein NA, Papke RL. Betel quid: New insights into an ancient addiction. Addict Biol. 2022 Sep;27(5):e13223. doi: 10.1111/adb.13223. PMID: 36001424; PMCID: PMC9552247.
  12. Aguirre RS, Kulkarni A, Becker MW, Lei X, Sarkar S, Ramanadham S, Phelps EA, Nakayasu ES, Sims EK, Mirmira RG. Extracellular vesicles in beta cell biology: Role of lipids in vesicle biogenesis, cargo, and intercellular signaling. Mol Metab. 2022 Sep;63:101545. doi: 10.1016/j.molmet.2022.101545. Epub 2022 Jul 8. PMID: 35817393; PMCID: PMC9294332.
  13. Dolan R, Lampejo AO, Santini-González J, Hodges NA, Phelps EA, Murfee WL. A Novel ex vivo Method for Investigating Vascularization of Transplanted Islets. J Vasc Res. 2022;59(4):229-238. doi: 10.1159/000523925. Epub 2022 Apr 22. PMID: 35462373; PMCID: PMC9308658.
  14. Jameson JF, Pacheco MO, Nguyen HH, Phelps EA, Stoppel WL. Recent Advances in Natural Materials for Corneal Tissue Engineering. Bioengineering (Basel). 2021 Oct 26;8(11):161. doi: 10.3390/bioengineering8110161. PMID: 34821727; PMCID: PMC8615221.
  15. Farhadi SA, Liu R, Becker MW, Phelps EA, Hudalla GA. Physical tuning of galectin-3 signaling. Proc Natl Acad Sci U S A. 2021 May 11;118(19):e2024117118. doi: 10.1073/pnas.2024117118. PMID: 33941700; PMCID: PMC8126866.
  16. Huber MK, Drotar DM, Hiller H, Beery ML, Joseph P, Kusmartseva I, Speier S, Atkinson MA, Mathews CE, Phelps EA. Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices. J Vis Exp. 2021 Apr 12;(170):10.3791/62207. doi: 10.3791/62207. PMID: 33900291; PMCID: PMC8314551.
  17. Santini-González J, Simonovich JA, Castro-Gutiérrez R, González-Vargas Y, Abuid NJ, Stabler CL, Russ HA, Phelps EA. In vitro generation of peri-islet basement membrane-like structures. Biomaterials. 2021 Jun;273:120808. doi: 10.1016/j.biomaterials.2021.120808. Epub 2021 Apr 13. PMID: 33895491; PMCID: PMC8131001.
  18. Ling KC, Hagan DW, Santini-González J, Phelps EA. Effects of sustained GABA releasing implants on pancreatic islets in mice. Drug Deliv Transl Res. 2021 Oct;11(5):2198-2208. doi: 10.1007/s13346-020-00886-2. Epub 2021 Jan 16. PMID: 33454926.
  19. Widener AE , Bhatta M , Angelini TE , Phelps EA . Guest-host interlinked PEG-MAL granular hydrogels as an engineered cellular microenvironment. Biomater Sci. 2021 Apr 7;9(7):2480-2493. doi: 10.1039/d0bm01499k. Epub 2021 Jan 12. PMID: 33432940.
  20. Fan J, Phelps EA. Career pathways, part 3. Nat Metab. 2021 Jan;3(1):2-4. doi: 10.1038/s42255-020-00302-6. PMID: 33398193.
  21. Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, Kang C, Sah R, Gaisano HY, Berggren PO, Baekkeskov S, Caicedo A, Phelps EA. Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell. Nat Metab. 2019 Nov;1(11):1110-1126. doi: 10.1038/s42255-019-0135-7. PMID: 32432213; PMCID: PMC7236889.
  22. Panzer JK, Hiller H, Cohrs CM, Almaça J, Enos SJ, Beery M, Cechin S, Drotar DM, Weitz JR, Santini J, Huber MK, Muhammad Fahd Qadir M, Pastori RL, Domínguez-Bendala J, Phelps EA, Atkinson MA, Pugliese A, Caicedo A, Kusmartseva I, Speier S. Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis. JCI Insight. 2020 Apr 23;5(8):e134525. doi: 10.1172/jci.insight.134525. PMID: 32324170; PMCID: PMC7205437.
  23. Becker MW, Simonovich JA, Phelps EA. Engineered microenvironments and microdevices for modeling the pathophysiology of type 1 diabetes. Biomaterials. 2019 Apr;198:49-62. doi: 10.1016/j.biomaterials.2018.07.002. Epub 2018 Jul 3. PMID: 30007472.
  24. Baekkeskov S, Hubbell JA, Phelps EA. Bioengineering strategies for inducing tolerance in autoimmune diabetes. Adv Drug Deliv Rev. 2017 May 15;114:256-265. doi: 10.1016/j.addr.2017.06.007. Epub 2017 Jun 15. PMID: 28625830.
  25. Rincon-Restrepo M, Mayer A, Hauert S, Bonner DK, Phelps EA, Hubbell JA, Swartz MA, Hirosue S. Vaccine nanocarriers: Coupling intracellular pathways and cellular biodistribution to control CD4 vs CD8 T cell responses. Biomaterials. 2017 Jul;132:48-58. doi: 10.1016/j.biomaterials.2017.03.047. Epub 2017 Mar 30. PMID: 28407494.
  26. Phelps EA, Cianciaruso C, Santo-Domingo J, Pasquier M, Galliverti G, Piemonti L, Berishvili E, Burri O, Wiederkehr A, Hubbell JA, Baekkeskov S. Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation. Sci Rep. 2017 Apr 12;7:45961. doi: 10.1038/srep45961. PMID: 28401888; PMCID: PMC5388888.
  27. Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Primary Human and Rat Beta-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Diabetes. 2017 Feb;66(2):460-473. doi: 10.2337/db16-0671. Epub 2016 Nov 21. PMID: 27872147.
  28. Phelps EA, Cianciaruso C, Michael IP, Pasquier M, Kanaani J, Nano R, Lavallard V, Billestrup N, Hubbell JA, Baekkeskov S. Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity. Diabetes. 2016 Sep;65(9):2686-99. doi: 10.2337/db16-0180. Epub 2016 Jun 9. PMID: 27284108; PMCID: PMC5001175.
  29. Brubaker CE, Velluto D, Demurtas D, Phelps EA, Hubbell JA. Crystalline Oligo(ethylene sulfide) Domains Define Highly Stable Supramolecular Block Copolymer Assemblies. ACS Nano. 2015 Jul 28;9(7):6872-81. doi: 10.1021/acsnano.5b02937. Epub 2015 Jul 8. PMID: 26125494.
  30. Phelps EA, Templeman KL, Thulé PM, García AJ. Engineered VEGF-releasing PEG-MAL hydrogel for pancreatic islet vascularization. Drug Deliv Transl Res. 2015 Apr;5(2):125-36. doi: 10.1007/s13346-013-0142-2. PMID: 25787738; PMCID: PMC4366610.
  31. Kanaani J, Cianciaruso C, Phelps EA, Pasquier M, Brioudes E, Billestrup N, Baekkeskov S. Compartmentalization of GABA synthesis by GAD67 differs between pancreatic beta cells and neurons. PLoS One. 2015 Feb 3;10(2):e0117130. doi: 10.1371/journal.pone.0117130. PMID: 25647668; PMCID: PMC4315522.
  32. Lee TT, García JR, Paez JI, Singh A, Phelps EA, Weis S, Shafiq Z, Shekaran A, Del Campo A, García AJ. Light-triggered in vivo activation of adhesive peptides regulates cell adhesion, inflammation and vascularization of biomaterials. Nat Mater. 2015 Mar;14(3):352-60. doi: 10.1038/nmat4157. Epub 2014 Dec 15. PMID: 25502097; PMCID: PMC4336636.
  33. Levit RD, Landázuri N, Phelps EA, Brown ME, García AJ, Davis ME, Joseph G, Long R, Safley SA, Suever JD, Lyle AN, Weber CJ, Taylor WR. Cellular encapsulation enhances cardiac repair. J Am Heart Assoc. 2013 Oct 10;2(5):e000367. doi: 10.1161/JAHA.113.000367. PMID: 24113327; PMCID: PMC3835246.
  34. Phelps EA, Headen DM, Taylor WR, Thulé PM, García AJ. Vasculogenic bio-synthetic hydrogel for enhancement of pancreatic islet engraftment and function in type 1 diabetes. Biomaterials. 2013 Jun;34(19):4602-11. doi: 10.1016/j.biomaterials.2013.03.012. Epub 2013 Mar 26. PMID: 23541111; PMCID: PMC3628538.
  35. Salimath AS, Phelps EA, Boopathy AV, Che PL, Brown M, García AJ, Davis ME. Dual delivery of hepatocyte and vascular endothelial growth factors via a protease-degradable hydrogel improves cardiac function in rats. PLoS One. 2012;7(11):e50980. doi: 10.1371/journal.pone.0050980. Epub 2012 Nov 30. PMID: 23226440; PMCID: PMC3511447.
  36. Phelps EA, Enemchukwu NO, Fiore VF, Sy JC, Murthy N, Sulchek TA, Barker TH, García AJ. Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery. Adv Mater. 2012 Jan 3;24(1):64-70, 2. doi: 10.1002/adma.201103574. Epub 2011 Dec 16. PMID: 22174081; PMCID: PMC3517145.
  37. Boerckel JD, Kolambkar YM, Dupont KM, Uhrig BA, Phelps EA, Stevens HY, García AJ, Guldberg RE. Effects of protein dose and delivery system on BMP-mediated bone regeneration. Biomaterials. 2011 Aug;32(22):5241-51. doi: 10.1016/j.biomaterials.2011.03.063. Epub 2011 Apr 20. PMID: 21507479; PMCID: PMC3129848.
  38. Phelps EA, García AJ. Engineering more than a cell: vascularization strategies in tissue engineering. Curr Opin Biotechnol. 2010 Oct;21(5):704-9. doi: 10.1016/j.copbio.2010.06.005. Epub 2010 Jul 16. PMID: 20638268; PMCID: PMC2952721.
  39. Sy JC, Phelps EA, García AJ, Murthy N, Davis ME. Surface functionalization of polyketal microparticles with nitrilotriacetic acid-nickel complexes for efficient protein capture and delivery. Biomaterials. 2010 Jun;31(18):4987-94. doi: 10.1016/j.biomaterials.2010.02.063. Epub 2010 Mar 25. PMID: 20346498; PMCID: PMC2866531.
  40. Phelps EA, Landázuri N, Thulé PM, Taylor WR, García AJ. Bioartificial matrices for therapeutic vascularization. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3323-8. doi: 10.1073/pnas.0905447107. Epub 2009 Dec 31. PMID: 20080569; PMCID: PMC2840448.
  41. Phelps EA, García AJ. Update on therapeutic vascularization strategies. Regen Med. 2009 Jan;4(1):65-80. doi: 10.2217/17460751.4.1.65. PMID: 19105617; PMCID: PMC2644334.