BMP2 repression and engineered oxygen control measures promote human mesenchymal stem cell recovery

Date(s) - 04/15/2013
4:00 pm - 5:15 pm

Dr. Nicholas R. Forsyth, Senior Lecturer in Stem Cell Biology, The Guy Hilton Research Laboratories, Keele University Medical School

The terms physiological normoxia, reduced oxygen, and hypoxia are all applied in association with studies investigating the culture of cells in an environment with lower oxygen tension than atmospheric (<21% O2). These studies are reliant on close control of O2 concentration in the surrounding environment. Exposure to ambient O2 (21%) concentrations for short or sustained periods of time can result in confounding results due to oxygen toxicity. Reduced O2 cell culture incubators and workstations are examples of adopted measures designed to reduce this oxygen toxicity and to promote consistency of observation. Human mesenchymal stem cells (hMSC) are multipotent progenitor cells isolated from multiple tissues but predominantly thought of as being bone marrow-derived. Physiological normoxia within the bone marrow is 2-8% O2.The wealth of experience surrounding hMSC culture and characterization has led to the emergence of a broad range of clinical trial applications.