Unraveling Sepsis Wound Healing: Insights from Immune Cell Dynamics and Molecular Signaling for Designing Therapeutic Interventions

Date(s) - 10/16/2023
3:00 pm - 4:00 pm

Communicore, C1-15

Leopold Green, Ph.D., Assistant Professor, Department of Biomedical Engineering, Purdue University

Immune response is critical in septic wound healing. The aberrant and imbalanced signaling dynamics primarily cause a dysfunctional innate immune response, exacerbating pathogen invasion of injured tissue and further stalling the healing process. To design biological controllers that regulate the critical divergence of the immune response during septicemia, we need to understand the intricate differences in immune cell dynamics and coordinated molecular signals of healthy and sepsis injury. Here, we deployed an ordinary differential equation (ODE)-based model to capture the hyper and hypo-inflammatory phases of sepsis wound healing. Our results indicate impaired macrophage polarization leads to a high abundance of monocytes, M1, and M2 macrophage phenotypes, resulting in immune paralysis. Using a model-based analysis framework, we designed a biological controller that successfully regulates macrophage dysregulation observed in septic wounds. Our model describes a systems biology approach to predict and explore critical parameters as potential therapeutic targets to transition septic wound inflammation toward a healthy, wound-healing state. Finally, I will share an overview of a design workflow incorporating systems biology, bacterial engineering, and DNA nanotechnology to build smart and tunable biocontrollers.


Dr. Green is a Synthetic Biologist with a diverse academic journey. He earned his bachelor’s degree in chemistry from Hampton University in 2011. With accolades, including the UNCF Merck Science Fellowship and Ronald McNair Fellowship, he pursued doctoral studies in Bioengineering at the University of California, Riverside. There, he was awarded the NSF Graduate Research Fellowship and focused on engineered nucleic acid-based nanostructures, coupling mechanical features with synthetic transcriptional oscillators. Completing his doctorate in 2017, he joined Caltech as a Post-doctoral Fellow, concentrating on engineering population controllers within synthetic E. coli-based communities. In the Fall of 2021, Dr. Green joined Purdue University’s Weldon School of Biomedical Engineering. His research centers on designing and building biological controllers as therapeutic agents for host-microbiome modulation, driven by his passion for addressing chronic inflammation and associated diseases.